Tony Spark 194 Questions 0 Answers 0 Best Answers 1k Points View Profile Tony SparkEnlightened Asked: April 25, 20202020-04-25T15:51:15+00:00 2020-04-25T15:51:15+00:00In: Biology questions and answers GeneRuler 1 kb Plus DNA Ladder bpng/0.5 ㎍ % 20000 20 4 10000 20 4 000 20 4 5000 75 15 4000 20 4 … 1 Answer Voted Oldest Recent Anonymous Added an answer on April 25, 2020 at 3:51 pm 2. A circular plasmid can produce 1 band on the gel if there is one recognition site of a restriction enzyme. A circular plasmid can produce 2 bands on the gel if there are two recognition site of a restriction enzyme. Since Ava I digestion produces two bands, the plasmid must contain two sites. Ava I produces a small band of 250 bp. 3. Since Eco RI digestion produces a single band, there must be a single site for Eco Ri on the plasmid. 4. Since Eco RI linearizes the plasmid, the size of the plasmid can be calculated by comparing the single band migration status with respect to the marker. Approximate size = 2500 bp – 3000 bp Double digestion produces 3 bands = 1500 + 1000 + 250 = 2750 bp Exact size of pUC19 = 2686 bp 5. See image. 0 Reply Share Share Share on Facebook Share on Twitter Share on LinkedIn Share on WhatsApp Leave an answerLeave an answerCancel reply Featured image Select file Browse Answer Anonymously Save my name, email, and website in this browser for the next time I comment.